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caspase 3 7 inhibitor i  (MedChemExpress)


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    MedChemExpress caspase 3 7 inhibitor i
    (A, B) LDH release, PI influx, and IL-1β release from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , and Gsdmd −/− primary or immortalized BMDMs (BMDMs and iBMDMs) after transfection of poly(dA:dT) in the absence or presence of the indicated inhibitors. (C, D, E) DNA cleavage, PI influx, and immunoblots showing <t>caspase-3/-7</t> processing from LPS-primed Gsdmd −/− BMDMs transfected with poly(dA:dT) or treated with 100 ng/ml TNF-α plus 10, 5, or 1 μM AZD5582 (extrinsic apoptosis) or 1 μM ABT-737 plus 10, 1, or 0.5 μM S63845 (intrinsic apoptosis). (F) Confocal images of LPS-primed Gsdmd −/− BMDMs transfected with poly(dA:dT) or left untreated and stained with CellTox Green (green). Scale bar = 10 μM. Graphs show mean ± SD. Data and blot are representative of at least three independent experiments.
    Caspase 3 7 Inhibitor I, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caspase 3 7 inhibitor i/product/MedChemExpress
    Average 90 stars, based on 1 article reviews
    caspase 3 7 inhibitor i - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Caspase-1 cleaves Bid to release mitochondrial SMAC and drive secondary necrosis in the absence of GSDMD"

    Article Title: Caspase-1 cleaves Bid to release mitochondrial SMAC and drive secondary necrosis in the absence of GSDMD

    Journal: Life Science Alliance

    doi: 10.26508/lsa.202000735

    (A, B) LDH release, PI influx, and IL-1β release from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , and Gsdmd −/− primary or immortalized BMDMs (BMDMs and iBMDMs) after transfection of poly(dA:dT) in the absence or presence of the indicated inhibitors. (C, D, E) DNA cleavage, PI influx, and immunoblots showing caspase-3/-7 processing from LPS-primed Gsdmd −/− BMDMs transfected with poly(dA:dT) or treated with 100 ng/ml TNF-α plus 10, 5, or 1 μM AZD5582 (extrinsic apoptosis) or 1 μM ABT-737 plus 10, 1, or 0.5 μM S63845 (intrinsic apoptosis). (F) Confocal images of LPS-primed Gsdmd −/− BMDMs transfected with poly(dA:dT) or left untreated and stained with CellTox Green (green). Scale bar = 10 μM. Graphs show mean ± SD. Data and blot are representative of at least three independent experiments.
    Figure Legend Snippet: (A, B) LDH release, PI influx, and IL-1β release from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , and Gsdmd −/− primary or immortalized BMDMs (BMDMs and iBMDMs) after transfection of poly(dA:dT) in the absence or presence of the indicated inhibitors. (C, D, E) DNA cleavage, PI influx, and immunoblots showing caspase-3/-7 processing from LPS-primed Gsdmd −/− BMDMs transfected with poly(dA:dT) or treated with 100 ng/ml TNF-α plus 10, 5, or 1 μM AZD5582 (extrinsic apoptosis) or 1 μM ABT-737 plus 10, 1, or 0.5 μM S63845 (intrinsic apoptosis). (F) Confocal images of LPS-primed Gsdmd −/− BMDMs transfected with poly(dA:dT) or left untreated and stained with CellTox Green (green). Scale bar = 10 μM. Graphs show mean ± SD. Data and blot are representative of at least three independent experiments.

    Techniques Used: Transfection, Western Blot, Staining, CellTox Assay

    PI influx from poly(dA:dT)–transfected LPS-primed Gsdmd −/− iBMDMs in the presence or absence of the indicated inhibitors added to the cells 30 min prior and during the experiment at the following concentrations: 50, 25, 12.5, and 6.25 μM VX765; 100, 50, 25, and 12.5 μM Caspase-3/7–specific inhibitor I; 30, 15, 7.5, and 3.25 μM K777; 100, 50, 25, and 12.5 μM PD 150606; 100, 50, 25, and 12.5 μM Calpeptin; 60, 30, 15, and 7.5 μM 7-Cl-O-Nec1; and 100, 50, 25, and 12.5 μM GSK872. Data and blot are representative of at least three independent experiments.
    Figure Legend Snippet: PI influx from poly(dA:dT)–transfected LPS-primed Gsdmd −/− iBMDMs in the presence or absence of the indicated inhibitors added to the cells 30 min prior and during the experiment at the following concentrations: 50, 25, 12.5, and 6.25 μM VX765; 100, 50, 25, and 12.5 μM Caspase-3/7–specific inhibitor I; 30, 15, 7.5, and 3.25 μM K777; 100, 50, 25, and 12.5 μM PD 150606; 100, 50, 25, and 12.5 μM Calpeptin; 60, 30, 15, and 7.5 μM 7-Cl-O-Nec1; and 100, 50, 25, and 12.5 μM GSK872. Data and blot are representative of at least three independent experiments.

    Techniques Used: Transfection

    (A, B) LDH release, caspase-3/-7 activity (DEVDase activity) and immunoblots showing caspase-3/-7 processing from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , and Gsdmd −/− primary BMDMs after transfection of poly(dA:dT). (C) LDH release from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , Gsdmd −/− , Gsdmd −/− /Casp3 −/− , Gsdmd −/− /Casp7 −/− , and Gsdmd −/− /Casp3 −/− /Casp7 −/− iBMDMs after transfection of poly(dA:dT). (C, D) Confocal images of cells from (C). Insets show membrane ballooning in dying cells at 3 h post-transfection. Scale bar = 10 μm. (E) Quantification of LDH release in LPS primed WT, Asc −/− , Casp-1 −/− /Casp-11 −/− , Gsdmd −/− , Gsdme −/− , and Gsdmd −/− /Gsdme −/− BMDMs transfected with poly(dA:dT) for 4 h. Graphs show mean ± SD. ** P ≤ 0.01, *** P ≤ 0.001, “ns,” no significance (unpaired t test). Data and blot are representative of at least three independent experiments.
    Figure Legend Snippet: (A, B) LDH release, caspase-3/-7 activity (DEVDase activity) and immunoblots showing caspase-3/-7 processing from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , and Gsdmd −/− primary BMDMs after transfection of poly(dA:dT). (C) LDH release from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , Gsdmd −/− , Gsdmd −/− /Casp3 −/− , Gsdmd −/− /Casp7 −/− , and Gsdmd −/− /Casp3 −/− /Casp7 −/− iBMDMs after transfection of poly(dA:dT). (C, D) Confocal images of cells from (C). Insets show membrane ballooning in dying cells at 3 h post-transfection. Scale bar = 10 μm. (E) Quantification of LDH release in LPS primed WT, Asc −/− , Casp-1 −/− /Casp-11 −/− , Gsdmd −/− , Gsdme −/− , and Gsdmd −/− /Gsdme −/− BMDMs transfected with poly(dA:dT) for 4 h. Graphs show mean ± SD. ** P ≤ 0.01, *** P ≤ 0.001, “ns,” no significance (unpaired t test). Data and blot are representative of at least three independent experiments.

    Techniques Used: Activity Assay, Western Blot, Transfection, Membrane

    Caspase-3 but not caspase-7 is required for lysis in Gsdmd −/− cel ls. (A) LDH release and caspase-3/-7 activity (DEVDase activity) from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , and Gsdmd −/− primary BMDMs after transfection of Salmonella typhimurium at MOI 10. (B) Immunoblots for caspase-3 and caspase-7 expression in lysates of Gsdmd −/− , Gsdmd −/− /Casp3 −/− , Gsdmd −/− /Casp7 −/− , and Gsdmd −/− /Casp3 −/− /Casp7 −/− iBMDMs. (C) PI influx from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , Gsdmd −/− , pool of Gsdmd −/− /Casp3 −/− clones (n = 3), pool of Gsdmd −/− /Casp7 −/− clones (n = 5), and Gsdmd −/− /Casp3 −/− /Casp7 −/− iBMDMs after transfection of poly(dA:dT). (D) Immunoblots showing caspase-3, caspase-7, and caspase-8 expression and LDH release after transfection poly(dA:dT) transfection from LPS-primed Gsdmd −/− iBMDMs transfected with control siRNA or siRNA targeting caspase-3, caspase-7, caspase-8 or both caspase-3/-7. Graphs show mean ± SD. ** P ≤ 0.01, *** P ≤ 0.001 (unpaired t test). Data and blot are representative of at least three independent experiments. Source data are available for this figure.
    Figure Legend Snippet: Caspase-3 but not caspase-7 is required for lysis in Gsdmd −/− cel ls. (A) LDH release and caspase-3/-7 activity (DEVDase activity) from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , and Gsdmd −/− primary BMDMs after transfection of Salmonella typhimurium at MOI 10. (B) Immunoblots for caspase-3 and caspase-7 expression in lysates of Gsdmd −/− , Gsdmd −/− /Casp3 −/− , Gsdmd −/− /Casp7 −/− , and Gsdmd −/− /Casp3 −/− /Casp7 −/− iBMDMs. (C) PI influx from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , Gsdmd −/− , pool of Gsdmd −/− /Casp3 −/− clones (n = 3), pool of Gsdmd −/− /Casp7 −/− clones (n = 5), and Gsdmd −/− /Casp3 −/− /Casp7 −/− iBMDMs after transfection of poly(dA:dT). (D) Immunoblots showing caspase-3, caspase-7, and caspase-8 expression and LDH release after transfection poly(dA:dT) transfection from LPS-primed Gsdmd −/− iBMDMs transfected with control siRNA or siRNA targeting caspase-3, caspase-7, caspase-8 or both caspase-3/-7. Graphs show mean ± SD. ** P ≤ 0.01, *** P ≤ 0.001 (unpaired t test). Data and blot are representative of at least three independent experiments. Source data are available for this figure.

    Techniques Used: Lysis, Activity Assay, Transfection, Western Blot, Expressing, Clone Assay, Control

    (A, B, C) Immunoblot showing Caspase-1 expression and caspase-3 processing, LDH release, and caspase-3/-7 activity (DEVDase activity) from LPS-primed Gsdmd −/− and Gsdmd −/− / Casp1 −/− immortalized BMDMs after transfection of poly(dA:dT). (D) PI influx of WT, Asc −/− , Casp1 −/− / Casp11 −/− , Casp1 −/− , Casp1 C284A/C284A , and Gsdmd −/− primary BMDMs after transfection of poly(dA:dT). Graphs show mean ± SD. ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001 (unpaired t test). Data and blot are representative of at least three independent experiments.
    Figure Legend Snippet: (A, B, C) Immunoblot showing Caspase-1 expression and caspase-3 processing, LDH release, and caspase-3/-7 activity (DEVDase activity) from LPS-primed Gsdmd −/− and Gsdmd −/− / Casp1 −/− immortalized BMDMs after transfection of poly(dA:dT). (D) PI influx of WT, Asc −/− , Casp1 −/− / Casp11 −/− , Casp1 −/− , Casp1 C284A/C284A , and Gsdmd −/− primary BMDMs after transfection of poly(dA:dT). Graphs show mean ± SD. ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001 (unpaired t test). Data and blot are representative of at least three independent experiments.

    Techniques Used: Western Blot, Expressing, Activity Assay, Transfection

    Gsdmd −/− /Bid −/− cells are resistant to canonical inflammasome activ ation. (A) Immunoblots showing Bid expression in Gsdmd −/− and Gsdmd −/− / Bid −/− iBMDMs. (B, C) Caspase-3/-7 activity (DEVDase activity) and PI influx in Gsdmd −/− and pool of Gsdmd −/− / Bid −/− clones (n = 5) iBMDMs after transfection with poly(dA:dT). (D) Confocal images of cells of Gsdmd −/− and Gsdmd −/− / Bid −/− iBMDMs after transfection with poly(dA:dT) for 3 h. Insets show membrane ballooning in dying cells. Scale bar = 10 μm. Graphs show mean ± SD. Data and blot are representative of at least three independent experiments.
    Figure Legend Snippet: Gsdmd −/− /Bid −/− cells are resistant to canonical inflammasome activ ation. (A) Immunoblots showing Bid expression in Gsdmd −/− and Gsdmd −/− / Bid −/− iBMDMs. (B, C) Caspase-3/-7 activity (DEVDase activity) and PI influx in Gsdmd −/− and pool of Gsdmd −/− / Bid −/− clones (n = 5) iBMDMs after transfection with poly(dA:dT). (D) Confocal images of cells of Gsdmd −/− and Gsdmd −/− / Bid −/− iBMDMs after transfection with poly(dA:dT) for 3 h. Insets show membrane ballooning in dying cells. Scale bar = 10 μm. Graphs show mean ± SD. Data and blot are representative of at least three independent experiments.

    Techniques Used: Western Blot, Expressing, Activity Assay, Clone Assay, Transfection, Membrane



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    Image Search Results


    (A, B) LDH release, PI influx, and IL-1β release from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , and Gsdmd −/− primary or immortalized BMDMs (BMDMs and iBMDMs) after transfection of poly(dA:dT) in the absence or presence of the indicated inhibitors. (C, D, E) DNA cleavage, PI influx, and immunoblots showing caspase-3/-7 processing from LPS-primed Gsdmd −/− BMDMs transfected with poly(dA:dT) or treated with 100 ng/ml TNF-α plus 10, 5, or 1 μM AZD5582 (extrinsic apoptosis) or 1 μM ABT-737 plus 10, 1, or 0.5 μM S63845 (intrinsic apoptosis). (F) Confocal images of LPS-primed Gsdmd −/− BMDMs transfected with poly(dA:dT) or left untreated and stained with CellTox Green (green). Scale bar = 10 μM. Graphs show mean ± SD. Data and blot are representative of at least three independent experiments.

    Journal: Life Science Alliance

    Article Title: Caspase-1 cleaves Bid to release mitochondrial SMAC and drive secondary necrosis in the absence of GSDMD

    doi: 10.26508/lsa.202000735

    Figure Lengend Snippet: (A, B) LDH release, PI influx, and IL-1β release from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , and Gsdmd −/− primary or immortalized BMDMs (BMDMs and iBMDMs) after transfection of poly(dA:dT) in the absence or presence of the indicated inhibitors. (C, D, E) DNA cleavage, PI influx, and immunoblots showing caspase-3/-7 processing from LPS-primed Gsdmd −/− BMDMs transfected with poly(dA:dT) or treated with 100 ng/ml TNF-α plus 10, 5, or 1 μM AZD5582 (extrinsic apoptosis) or 1 μM ABT-737 plus 10, 1, or 0.5 μM S63845 (intrinsic apoptosis). (F) Confocal images of LPS-primed Gsdmd −/− BMDMs transfected with poly(dA:dT) or left untreated and stained with CellTox Green (green). Scale bar = 10 μM. Graphs show mean ± SD. Data and blot are representative of at least three independent experiments.

    Article Snippet: VX-765 (MedChemExpress), Caspase-3/7 inhibitor I (CAS 220509-74-0; Santa Cruz Biotechnology), Q-VD-Oph (Selleck Chemicals), AZD5582 (Selleck Chemicals), 7-Cl-O-Nec1 (Abcam), GSK872 (Selleck Chemicals), K777 (AdipoGen), PD 150606 (Tocris), Calpeptin (Selleck Chemicals), ABT-737 (Selleck Chemicals), S63845 (Selleck Chemicals), and Nigericin (InvivoGen).

    Techniques: Transfection, Western Blot, Staining, CellTox Assay

    PI influx from poly(dA:dT)–transfected LPS-primed Gsdmd −/− iBMDMs in the presence or absence of the indicated inhibitors added to the cells 30 min prior and during the experiment at the following concentrations: 50, 25, 12.5, and 6.25 μM VX765; 100, 50, 25, and 12.5 μM Caspase-3/7–specific inhibitor I; 30, 15, 7.5, and 3.25 μM K777; 100, 50, 25, and 12.5 μM PD 150606; 100, 50, 25, and 12.5 μM Calpeptin; 60, 30, 15, and 7.5 μM 7-Cl-O-Nec1; and 100, 50, 25, and 12.5 μM GSK872. Data and blot are representative of at least three independent experiments.

    Journal: Life Science Alliance

    Article Title: Caspase-1 cleaves Bid to release mitochondrial SMAC and drive secondary necrosis in the absence of GSDMD

    doi: 10.26508/lsa.202000735

    Figure Lengend Snippet: PI influx from poly(dA:dT)–transfected LPS-primed Gsdmd −/− iBMDMs in the presence or absence of the indicated inhibitors added to the cells 30 min prior and during the experiment at the following concentrations: 50, 25, 12.5, and 6.25 μM VX765; 100, 50, 25, and 12.5 μM Caspase-3/7–specific inhibitor I; 30, 15, 7.5, and 3.25 μM K777; 100, 50, 25, and 12.5 μM PD 150606; 100, 50, 25, and 12.5 μM Calpeptin; 60, 30, 15, and 7.5 μM 7-Cl-O-Nec1; and 100, 50, 25, and 12.5 μM GSK872. Data and blot are representative of at least three independent experiments.

    Article Snippet: VX-765 (MedChemExpress), Caspase-3/7 inhibitor I (CAS 220509-74-0; Santa Cruz Biotechnology), Q-VD-Oph (Selleck Chemicals), AZD5582 (Selleck Chemicals), 7-Cl-O-Nec1 (Abcam), GSK872 (Selleck Chemicals), K777 (AdipoGen), PD 150606 (Tocris), Calpeptin (Selleck Chemicals), ABT-737 (Selleck Chemicals), S63845 (Selleck Chemicals), and Nigericin (InvivoGen).

    Techniques: Transfection

    (A, B) LDH release, caspase-3/-7 activity (DEVDase activity) and immunoblots showing caspase-3/-7 processing from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , and Gsdmd −/− primary BMDMs after transfection of poly(dA:dT). (C) LDH release from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , Gsdmd −/− , Gsdmd −/− /Casp3 −/− , Gsdmd −/− /Casp7 −/− , and Gsdmd −/− /Casp3 −/− /Casp7 −/− iBMDMs after transfection of poly(dA:dT). (C, D) Confocal images of cells from (C). Insets show membrane ballooning in dying cells at 3 h post-transfection. Scale bar = 10 μm. (E) Quantification of LDH release in LPS primed WT, Asc −/− , Casp-1 −/− /Casp-11 −/− , Gsdmd −/− , Gsdme −/− , and Gsdmd −/− /Gsdme −/− BMDMs transfected with poly(dA:dT) for 4 h. Graphs show mean ± SD. ** P ≤ 0.01, *** P ≤ 0.001, “ns,” no significance (unpaired t test). Data and blot are representative of at least three independent experiments.

    Journal: Life Science Alliance

    Article Title: Caspase-1 cleaves Bid to release mitochondrial SMAC and drive secondary necrosis in the absence of GSDMD

    doi: 10.26508/lsa.202000735

    Figure Lengend Snippet: (A, B) LDH release, caspase-3/-7 activity (DEVDase activity) and immunoblots showing caspase-3/-7 processing from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , and Gsdmd −/− primary BMDMs after transfection of poly(dA:dT). (C) LDH release from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , Gsdmd −/− , Gsdmd −/− /Casp3 −/− , Gsdmd −/− /Casp7 −/− , and Gsdmd −/− /Casp3 −/− /Casp7 −/− iBMDMs after transfection of poly(dA:dT). (C, D) Confocal images of cells from (C). Insets show membrane ballooning in dying cells at 3 h post-transfection. Scale bar = 10 μm. (E) Quantification of LDH release in LPS primed WT, Asc −/− , Casp-1 −/− /Casp-11 −/− , Gsdmd −/− , Gsdme −/− , and Gsdmd −/− /Gsdme −/− BMDMs transfected with poly(dA:dT) for 4 h. Graphs show mean ± SD. ** P ≤ 0.01, *** P ≤ 0.001, “ns,” no significance (unpaired t test). Data and blot are representative of at least three independent experiments.

    Article Snippet: VX-765 (MedChemExpress), Caspase-3/7 inhibitor I (CAS 220509-74-0; Santa Cruz Biotechnology), Q-VD-Oph (Selleck Chemicals), AZD5582 (Selleck Chemicals), 7-Cl-O-Nec1 (Abcam), GSK872 (Selleck Chemicals), K777 (AdipoGen), PD 150606 (Tocris), Calpeptin (Selleck Chemicals), ABT-737 (Selleck Chemicals), S63845 (Selleck Chemicals), and Nigericin (InvivoGen).

    Techniques: Activity Assay, Western Blot, Transfection, Membrane

    Caspase-3 but not caspase-7 is required for lysis in Gsdmd −/− cel ls. (A) LDH release and caspase-3/-7 activity (DEVDase activity) from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , and Gsdmd −/− primary BMDMs after transfection of Salmonella typhimurium at MOI 10. (B) Immunoblots for caspase-3 and caspase-7 expression in lysates of Gsdmd −/− , Gsdmd −/− /Casp3 −/− , Gsdmd −/− /Casp7 −/− , and Gsdmd −/− /Casp3 −/− /Casp7 −/− iBMDMs. (C) PI influx from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , Gsdmd −/− , pool of Gsdmd −/− /Casp3 −/− clones (n = 3), pool of Gsdmd −/− /Casp7 −/− clones (n = 5), and Gsdmd −/− /Casp3 −/− /Casp7 −/− iBMDMs after transfection of poly(dA:dT). (D) Immunoblots showing caspase-3, caspase-7, and caspase-8 expression and LDH release after transfection poly(dA:dT) transfection from LPS-primed Gsdmd −/− iBMDMs transfected with control siRNA or siRNA targeting caspase-3, caspase-7, caspase-8 or both caspase-3/-7. Graphs show mean ± SD. ** P ≤ 0.01, *** P ≤ 0.001 (unpaired t test). Data and blot are representative of at least three independent experiments. Source data are available for this figure.

    Journal: Life Science Alliance

    Article Title: Caspase-1 cleaves Bid to release mitochondrial SMAC and drive secondary necrosis in the absence of GSDMD

    doi: 10.26508/lsa.202000735

    Figure Lengend Snippet: Caspase-3 but not caspase-7 is required for lysis in Gsdmd −/− cel ls. (A) LDH release and caspase-3/-7 activity (DEVDase activity) from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , and Gsdmd −/− primary BMDMs after transfection of Salmonella typhimurium at MOI 10. (B) Immunoblots for caspase-3 and caspase-7 expression in lysates of Gsdmd −/− , Gsdmd −/− /Casp3 −/− , Gsdmd −/− /Casp7 −/− , and Gsdmd −/− /Casp3 −/− /Casp7 −/− iBMDMs. (C) PI influx from LPS-primed WT, Asc −/− , Casp1 −/− / Casp11 −/− , Gsdmd −/− , pool of Gsdmd −/− /Casp3 −/− clones (n = 3), pool of Gsdmd −/− /Casp7 −/− clones (n = 5), and Gsdmd −/− /Casp3 −/− /Casp7 −/− iBMDMs after transfection of poly(dA:dT). (D) Immunoblots showing caspase-3, caspase-7, and caspase-8 expression and LDH release after transfection poly(dA:dT) transfection from LPS-primed Gsdmd −/− iBMDMs transfected with control siRNA or siRNA targeting caspase-3, caspase-7, caspase-8 or both caspase-3/-7. Graphs show mean ± SD. ** P ≤ 0.01, *** P ≤ 0.001 (unpaired t test). Data and blot are representative of at least three independent experiments. Source data are available for this figure.

    Article Snippet: VX-765 (MedChemExpress), Caspase-3/7 inhibitor I (CAS 220509-74-0; Santa Cruz Biotechnology), Q-VD-Oph (Selleck Chemicals), AZD5582 (Selleck Chemicals), 7-Cl-O-Nec1 (Abcam), GSK872 (Selleck Chemicals), K777 (AdipoGen), PD 150606 (Tocris), Calpeptin (Selleck Chemicals), ABT-737 (Selleck Chemicals), S63845 (Selleck Chemicals), and Nigericin (InvivoGen).

    Techniques: Lysis, Activity Assay, Transfection, Western Blot, Expressing, Clone Assay, Control

    (A, B, C) Immunoblot showing Caspase-1 expression and caspase-3 processing, LDH release, and caspase-3/-7 activity (DEVDase activity) from LPS-primed Gsdmd −/− and Gsdmd −/− / Casp1 −/− immortalized BMDMs after transfection of poly(dA:dT). (D) PI influx of WT, Asc −/− , Casp1 −/− / Casp11 −/− , Casp1 −/− , Casp1 C284A/C284A , and Gsdmd −/− primary BMDMs after transfection of poly(dA:dT). Graphs show mean ± SD. ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001 (unpaired t test). Data and blot are representative of at least three independent experiments.

    Journal: Life Science Alliance

    Article Title: Caspase-1 cleaves Bid to release mitochondrial SMAC and drive secondary necrosis in the absence of GSDMD

    doi: 10.26508/lsa.202000735

    Figure Lengend Snippet: (A, B, C) Immunoblot showing Caspase-1 expression and caspase-3 processing, LDH release, and caspase-3/-7 activity (DEVDase activity) from LPS-primed Gsdmd −/− and Gsdmd −/− / Casp1 −/− immortalized BMDMs after transfection of poly(dA:dT). (D) PI influx of WT, Asc −/− , Casp1 −/− / Casp11 −/− , Casp1 −/− , Casp1 C284A/C284A , and Gsdmd −/− primary BMDMs after transfection of poly(dA:dT). Graphs show mean ± SD. ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001 (unpaired t test). Data and blot are representative of at least three independent experiments.

    Article Snippet: VX-765 (MedChemExpress), Caspase-3/7 inhibitor I (CAS 220509-74-0; Santa Cruz Biotechnology), Q-VD-Oph (Selleck Chemicals), AZD5582 (Selleck Chemicals), 7-Cl-O-Nec1 (Abcam), GSK872 (Selleck Chemicals), K777 (AdipoGen), PD 150606 (Tocris), Calpeptin (Selleck Chemicals), ABT-737 (Selleck Chemicals), S63845 (Selleck Chemicals), and Nigericin (InvivoGen).

    Techniques: Western Blot, Expressing, Activity Assay, Transfection

    Gsdmd −/− /Bid −/− cells are resistant to canonical inflammasome activ ation. (A) Immunoblots showing Bid expression in Gsdmd −/− and Gsdmd −/− / Bid −/− iBMDMs. (B, C) Caspase-3/-7 activity (DEVDase activity) and PI influx in Gsdmd −/− and pool of Gsdmd −/− / Bid −/− clones (n = 5) iBMDMs after transfection with poly(dA:dT). (D) Confocal images of cells of Gsdmd −/− and Gsdmd −/− / Bid −/− iBMDMs after transfection with poly(dA:dT) for 3 h. Insets show membrane ballooning in dying cells. Scale bar = 10 μm. Graphs show mean ± SD. Data and blot are representative of at least three independent experiments.

    Journal: Life Science Alliance

    Article Title: Caspase-1 cleaves Bid to release mitochondrial SMAC and drive secondary necrosis in the absence of GSDMD

    doi: 10.26508/lsa.202000735

    Figure Lengend Snippet: Gsdmd −/− /Bid −/− cells are resistant to canonical inflammasome activ ation. (A) Immunoblots showing Bid expression in Gsdmd −/− and Gsdmd −/− / Bid −/− iBMDMs. (B, C) Caspase-3/-7 activity (DEVDase activity) and PI influx in Gsdmd −/− and pool of Gsdmd −/− / Bid −/− clones (n = 5) iBMDMs after transfection with poly(dA:dT). (D) Confocal images of cells of Gsdmd −/− and Gsdmd −/− / Bid −/− iBMDMs after transfection with poly(dA:dT) for 3 h. Insets show membrane ballooning in dying cells. Scale bar = 10 μm. Graphs show mean ± SD. Data and blot are representative of at least three independent experiments.

    Article Snippet: VX-765 (MedChemExpress), Caspase-3/7 inhibitor I (CAS 220509-74-0; Santa Cruz Biotechnology), Q-VD-Oph (Selleck Chemicals), AZD5582 (Selleck Chemicals), 7-Cl-O-Nec1 (Abcam), GSK872 (Selleck Chemicals), K777 (AdipoGen), PD 150606 (Tocris), Calpeptin (Selleck Chemicals), ABT-737 (Selleck Chemicals), S63845 (Selleck Chemicals), and Nigericin (InvivoGen).

    Techniques: Western Blot, Expressing, Activity Assay, Clone Assay, Transfection, Membrane